Galectin 3 Rabbit Polyclonal Antibody
| Cat Number: | AB-82806 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Full length recombinant galectin 3 expressed in and purified from E. coli.. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1/500 – 1/5000 |
| Molecular Weight: | 30kD |
| Purification: | Serum |
| Synonyms: | LGALS3; MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; |
| Background: | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants. |
| Form: | Liquid |
| Buffer: | Supplied as an aliquot of serum plus 5mM NaN3 |
| Storage: | Store at 4°C for short term, for longer term store at -20°C + avoid freeze/thaw cycles |
Western blot analysis of different tissue and cell lysates using rabbit pAb to galectin 3, dilution 1:5,000 in green:
[1] protein standard and mouse tissue lysates:
[2] heart,
[3] liver,
[4] kidney,
[5] lung, [6] rat cortical neuron-glial primary cell culture lysate and
[7] pig spinal cord lysate. The band at about 30kDa corresponds to the galectin 3 protein.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to galectin 3, dilution 1:2,000 in green, and costained with mouse mAb to GFAP,dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. Certain glial cells express only galectin-3 protein, and appear green, while the majority of glial cells and astrocytes produces GFAP protein and so appear red, a few cells that express both protein appear orange-yellow.