GAPDH Rabbit Polyclonal Antibody
| Cat Number: | GB15004-100 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 μL |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB 1: 3000-1: 5000 IHC/IF 1: 500-1: 2000 ICC/IF 1: 500-1: 1000 |
| Molecular: | 36 kDa / 36 kDa |
| Purification: | Affinity purification |
| Synonyms: | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Background: | Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed. |
| Form: | Liquid |
| Buffer: | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
| Storage: | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
WB analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.
WB analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.
IHC analysis of GAPDH. Sample: Human esophageal cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.