GFAP Rabbit Polyclonal Antibody
| Cat Number: | ABE5510 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Clone: | Polyclonal |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GFAP. AA range:11-60 |
| Reactivity: | Human;Rat;Mouse; |
| Applications: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Molecular: | 50kD |
| Purification: | The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | GFAP; Glial fibrillary acidic protein; GFAP |
| Background: | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008], |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide |
| Storage: | -20°C/1 year |
Immunofluorescence analysis of Hela cell. 1. GFAP Polyclonal Antibody(green) was diluted at 1:200 (4° overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3. DAPI(blue) 10min.
Western Blot analysis of RAT-MUSCLE cells using GFAP Polyclonal Antibody diluted at 1:2000
Western Blot analysis of RAW using GFAP Polyclonal Antibody diluted at 1:2000