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Glutamate receptor 1 Rabbit Polyclonal Antibody

Cat Number: AB-J8091
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human GluR1. AA range:816-865
Reactivity: Human;Mouse;Rat
Applications:

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300
Immunofluorescence: 1/200 – 1/1000

Molecular Weight: 95kDa
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms: GRIA1; GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GluA1
Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole
propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Form: Liquid
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-J8091-1

Western Blot analysis of various cells using GluR-1 Polyclonal Antibody diluted at 1:500

AB-J8091-2

Western Blot analysis of HELA HEPG2 using GluR-1 Polyclonal Antibody diluted at 1:500

AB-J8091-3

Immunofluorescence analysis of Hela cell. 1,GluR-1 Polyclonal Antibody(green) was diluted at 1:200(4°overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.

AB-J8091-4

Immunofluorescence analysis of HeLa cells, using GluR1 Antibody. The picture on the right is blocked with the synthesized peptide.