GOLPH3
| Cat Number: | AB-84328 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human GOLPH3. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 37kDa |
| Purification: | Aff. Pur. |
| Synonyms: | GOLPH3;GOPP1;GPP34;MIDAS;Vps74 |
| Background: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using GOLPH3 antibody at 1:3000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 15s.
Immunohistochemistry Paraffin-embedded human prostate cancer tissue stained for GOLPH3/MIDAS using at 1:100 dilution in immunohistochemical analysis.
Immunofluorescence analysis of L929 cells using GOLPH3 antibody at
dilution of 1:100. Blue: DAPI for nuclear staining.