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GOLPH3

Cat Number: AB-84328
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human GOLPH3.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:100 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 37kDa
Purification: Aff. Pur.
Synonyms: GOLPH3;GOPP1;GPP34;MIDAS;Vps74
Background:

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84328-1.jpg

Western blot analysis of extracts of various cell lines, using GOLPH3 antibody at 1:3000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 15s.

AB-84328-2.jpg

Immunohistochemistry Paraffin-embedded human prostate cancer tissue stained for GOLPH3/MIDAS using at 1:100 dilution in immunohistochemical analysis.

AB-84328-3.jpg

Immunofluorescence analysis of L929 cells using GOLPH3 antibody at
dilution of 1:100. Blue: DAPI for nuclear staining.