GRP78 BiP rabbit pAb
| Cat Number: | ABE8607 |
|---|---|
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Clone: | Polyclonal |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide from human protein at AA range: 505-570 |
| Reactivity: | Human;Mouse;Rat;Fish |
| Applications: | Western Blot: 1/500 - 1/2000.IHC-p/f:1:50-300. ELISA 1:10000-20000 |
| Molecular: | 78kD |
| Synonyms: | 78 kDa glucose-regulated protein (GRP-78) (Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78) (Heat shock 70 kDa protein 5) (Immunoglobulin heavy chain-binding protein) (BiP) |
| Background: | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010], |
| Form: | liquid |
| Storage: | -20°C/1 year |
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) – Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200