HER1 / EGFR
| Cat Number: | MAB-94446 |
|---|---|
| Size: | 100 ug |
| Clone: | IS-23 |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG2b |
| Immunogen: | A synthesized peptide derived from human EGFR (ErbB 1) Antibody EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGFalpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 |
| Molecular Weight: | 175kDa |
| Purification: | Affinity-chromatography |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle |
Western blot analysis of extracts of OVCAR3 cells, using EGFR Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
IHC analysis of EGFR using anti-EGFR antibody on paraffin-embedded human stomach cancer.
EGFR was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Immunofluorescence analysis of HeLa cells using EGFR antibody at dilution
of 1:100.
Blue: DAPI for nuclear staining.