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HER1 / EGFR

Cat Number: MAB-94446
Size: 100 ug
Clone: IS-23
Concentration: 1mg/ml
Host: Ms
Isotype: IgG2b
Immunogen: A synthesized peptide derived from human EGFR (ErbB 1) Antibody EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGFalpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:50- 1:200
Immunofluorescence: 1:50-1:200
Immunoprecipitation: 1:50-1:200

Molecular: 175kDa
Purification: Affinity-chromatography
Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle
MAB-94446-1.jpg

Western blot analysis of extracts of OVCAR3 cells, using EGFR Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

MAB-94446-2.jpg

IHC analysis of EGFR using anti-EGFR antibody on paraffin-embedded human stomach cancer.
EGFR was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

MAB-94446-3.jpg

Immunofluorescence analysis of HeLa cells using EGFR antibody at dilution
of 1:100.
Blue: DAPI for nuclear staining.