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HIF-1alpha Rabbit Polyclonal Antibody

Cat Number: AB-80484
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant Protein
Reactivity: Human, Mouse, Rat
Applications:

WesternBlot:1/500-1000
Immunohistochemistry:1/100-200

Molecular Weight: Observed MW band: 90-130 kDa
Purification: Aff. Pur.
Synonyms: Hypoxia inducibel factor 1 alpha, HIF1A, ARNT, MOP1, PAS domaincontaining protein 8, PASD8
Background:

Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a protein that in humans is encoded by the HIF1A gene. HIF-1-alpha found in mammalian cells growing at low oxygen concentrations. It plays an essential role in cellular and systemic responses to hypoxia.

Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-80484-1

Western blot analysis of 1) Mouse Liver Tissue Lysate, 2)Rat Liver Tissue Lysate, 3) HepG2 Cell Lysate using HIF-1α Rabbit pAb diluted at 1:1000.

AB-80484-2

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HIF-1α Rabbit pAb diluted at 1:200