|Immunogen:||Recombinant protein of human HIF1α.|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:500 – 1:2000
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20°C, and avoid repeat freeze-thaw cycles.|
Western blot analysis of extracts of
mouse kidney, using HIF1α antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
Detection: ECL West Pico Plus.
Exposure time: 5s.
Immunohistochemistry of paraffin
Embedded human liver cancer using
HIF1α antibody at dilution
of 1:200 (40x lens).