HIS Tag
| Cat Number: | MAB-91879 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | B8 |
| Concentration: | 1mg/mL |
| Host: | Ms |
| Isotype: | IgG1 |
| Immunogen: | A synthetic peptide of His tag |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:2000 – 1:5000 |
| Molecular Weight: | 30kDa/38kDa/55kDa |
| Purification: | Affinity purification |
| Background: | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody at 1:20000 dilution.
Secondary antibody: HRP Goat Anti- Mouse IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.
Western blot analysis of extracts of normal 293T cells and 293T transfected
with His-CEND1 protein,using His-Tag
antibody at 1:20000 dilution.
Secondary antibody: HRP Goat Anti- Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Exposure time: 90s.
Immunoprecipitation of over-expressed
His-tagged protein in 293T cells
incubated using His-tag antibody
A mock served as negative
control and over-expressed 293T cell
lysate served as positive control.
Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb
Blue: DAPI for nuclear staining.