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HIS Tag

Cat Number: MAB-91879
Conjugate: Unconjugated
Size: 100 ug
Clone: B8
Concentration: 1mg/mL
Host: Ms
Isotype: IgG1
Immunogen: A synthetic peptide of His tag
Reactivity: All Species
Applications:

Western Blot: 1:2000 – 1:5000
Immunofluorescence: 1:50 – 1:200
Immunoprecipitation: 1:50 – 1:100

Molecular: 30kDa/38kDa/55kDa
Purification: Affinity purification
Background:

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-91879-1.jpg

Western blot analysis of extracts of
normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody at 1:20000 dilution.
Secondary antibody: HRP Goat Anti- Mouse IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.

MAB-91879-2.jpg

Western blot analysis of extracts of normal 293T cells and 293T transfected
with His-CEND1 protein,using His-Tag
antibody at 1:20000 dilution.
Secondary antibody: HRP Goat Anti- Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Exposure time: 90s.

MAB-91879-3.jpg

Immunoprecipitation of over-expressed
His-tagged protein in 293T cells
incubated using His-tag antibody
A mock served as negative
control and over-expressed 293T cell
lysate served as positive control.

MAB-91879-4.jpg

Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb
Blue: DAPI for nuclear staining.