HIS Tag Mouse Monoclonal Antibody
| Cat Number: | MAB-91879 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | B8 |
| Concentration: | 1mg/mL |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | A synthetic peptide corresponding to His tag. |
| Reactivity: | All Species |
| Applications: | Western Blot: 1:5000-1:20000 |
| Molecular Weight: | 27kDa/35kDa |
| Purification: | Affinity purification |
| Synonyms: | His;His tag;His-tag |
| Background: | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates, using His-Tag mAb at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb
Blue: DAPI for nuclear staining.