HIS Tag Mouse Monoclonal Antibody

Cat Number: MAB-91879
Conjugate: Unconjugated
Size: 100 ug
Clone: B8
Concentration: 1mg/mL
Host: Mouse
Isotype: IgG1
Immunogen: A synthetic peptide corresponding to His tag.
Reactivity: All Species

Western Blot: 1:5000-1:20000
Immunofluorescence: 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Immunoprecipitation: 1:50 – 1:200

Molecular Weight: 27kDa/35kDa
Purification: Affinity purification
Synonyms: His;His tag;His-tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can
be detected by using His antibodies.

Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of various lysates, using His-Tag mAb at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.


Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb
Blue: DAPI for nuclear staining.