HIS Tag
Cat Number: | MAB-91879 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | B8 |
Concentration: | 1mg/mL |
Host: | Ms |
Isotype: | IgG1 |
Immunogen: | A synthetic peptide of His tag |
Reactivity: | All Species |
Applications: | Western Blot: 1:2000 – 1:5000 |
Molecular Weight: | 30kDa/38kDa/55kDa |
Purification: | Affinity purification |
Background: | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies. |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |

Western blot analysis of extracts of
normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody at 1:20000 dilution.
Secondary antibody: HRP Goat Anti- Mouse IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3s.

Western blot analysis of extracts of normal 293T cells and 293T transfected
with His-CEND1 protein,using His-Tag
antibody at 1:20000 dilution.
Secondary antibody: HRP Goat Anti- Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus Exposure time: 90s.

Immunoprecipitation of over-expressed
His-tagged protein in 293T cells
incubated using His-tag antibody
A mock served as negative
control and over-expressed 293T cell
lysate served as positive control.

Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb
Blue: DAPI for nuclear staining.