HSP70-2 Rabbit Polyclonal Antibody
Cat Number: | AB-83532 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-639 of human HSP70-2 |
Reactivity: | Human, Mouse, Rat |
Applications: | Western Blot 1:500 – 1:2000 |
Molecular Weight: | 75kDa |
Purification: | Affinity Purified |
Synonyms: | HSPA2;HSP70-3 |
Background: | Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |

Western blot analysis of extracts of various cell lines, using HSP70-2 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Immunofluorescence analysis of L929 cells
using HSP70-2 antibody at dilution
of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS
cells using HSP70-2 antibody at
dilution of 1:100. Blue: DAPI for nuclear
staining.