IGF1R Rabbit Polyclonal Antibody
| Cat Number: | AB-81364 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1201-1367 of human IGF1R. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:1000 – 1:5000 |
| Molecular Weight: | 100kDa |
| Purification: | Affinity purification |
| Synonyms: | IGFR; CD221; IGFIR; JTK13; 1R |
| Background: | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | At 4°C for one month. Or store frozen at -20°C for a longer time. Avoid freezing and thawing. |
Western blot analysis of lysates from wild type (WT) and IGF1R knockdown (KD) 293T cells using
[KDValidated] IGF1R Rabbit pAb at 1:3000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Western blot analysis of various lysates using [KD Validated] IGF1R Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.