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IGF1R

Cat Number: AB-81364
Conjugate: Unconjugated
Size: 100μg
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor(1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid.
Reactivity: Hu, Ms, Rt
Applications:

Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/ml (by heat)
Western blot: 0.1-0.5 μg/ml

Molecular: 154kDa
Purification: Aff. Pur.
Background:

IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults – meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of hosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase -meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-Insulin Receptor heterodimers. It does not react with IRS1 (insulin receptor substrate 1).Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney. .

Form: Liquid
Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Storage: At 4°C for one month. Or store frozen at -20°C for a longer time. Avoid freezing and thawing.
AB-81364-1.jpg

Anti-IGF1 Receptor antibody, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: MCF-7 Cell Lysate

AB-81364-2.jpg

Anti-IGF1 Receptor antibody, IHC(P)
IHC(P): Rat Pancreas Tissue