IL-6 Rabbit Polyclonal Antibody
| Cat Number: | ABN04151 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant protein of human IL6. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200 |
| Molecular: | 24 kDa |
| Purification: | Affinity purification |
| Synonyms: | IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
| Background: | Interleukine 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts and epithelial cells. It has a wide variety of biological functions through activating a cellsurface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. The cytokine plays an essential role in the final differentiation of b-cells into Igsecreting cells, and induces myeloma, plasmacytoma growth, nerve cells differentiations. In addition, IL-6 is an important regulator of acute phase reaction. |
| Form: | liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% sodium azide, pH 7.3. |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Western blot analysis of IL-6 in various cell lines lysates using IL-6 antibody.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using IL-6
antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for
antigen retrieval.
Immunofluorescence analysis of IL-6 in RAW264.7 cells using IL-6 antibody,and
DAPI (blue).