|Immunogen:||A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ITGB1|
|Reactivity:||Hu, Ms, Rt|
Western Blot: 1:500 – 1:2000
|Synonyms:||ITGB1; CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BET A; VLAB; integrin beta-1|
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of various cell lines, using ITGB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric cancer using ITGB1 antibody at dilution of 1:100 (40x lens).