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Integrin B1

Cat Number: AB-84115
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ITGB1
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 -1:200
Immunofluorescence: 1:50 -1:200

Molecular: 100-135kDa
Purification: Aff. Pur.
Synonyms: ITGB1; CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BET A; VLAB; integrin beta-1
Background:

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84115-1.jpg

Western blot analysis of extracts of various cell lines, using ITGB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 90s.

AB-84115-2.jpg

Immunohistochemistry of paraffin-embedded human gastric cancer using ITGB1 antibody at dilution of 1:100 (40x lens).