Interleukin-1 Beta Rabbit Monoclonal Antibody
| Cat Number: | MAB-94634 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | MI1 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230 |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 17kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B |
| Background: | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of THP-1 cells, using IL1 beta antibody at 1:1000 dilution.THP-1 cells were treated by LPS (1 μg/mL) at 37℃ for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.
Exposure time: 3min.
Immunofluorescence analysis of THP-1
cells using IL1 beta antibody.
(THP-1 cells were treated by LPS
(1 μg/mL) at 37℃ for 8 hours.
Blue: DAPI for nuclear staining.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,IL-1β Polyclonal Antibody was
diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by
secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,IL-1β Polyclonal Antibody was diluted at
1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).
3,Secondary antibody was diluted at 1:200(room temperture).
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue.
1,IL-1β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).3,Secondary antibody was diluted at 1:200(roomtempeRature, 30min). Negative control was used by
secondary antibody only.