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Interleukin-1 Beta

Cat Number: MAB-94634
Conjugate: Unconjugated
Size: 100 ug
Clone: MI1
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthesized peptide derived from human IL1 beta.
Reactivity: Hu
Applications:

Western Blot: 1:500 – 1:2000
Immunofluorescence: 1:50 – 1:200

Molecular: 35kDa
Purification: Aff. Pur.
Synonyms: IL-1;IL1-BETA;IL1F2;IL1 beta;IL1B
Background:

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol, pH7.3
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-94634-1.jpg

Western blot analysis of extracts of THP-1
cells, using IL1 beta antibody at 1:1000
dilution.THP-1 cells were treated by
LPS (1 μg/mL) at 37℃ for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit
IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.

MAB-94634-2.jpg

Immunofluorescence analysis of THP-1
cells using IL1 beta antibody.
(THP-1 cells were treated by LPS
(1 μg/mL) at 37℃ for 8 hours.
Blue: DAPI for nuclear staining.

References

References for IL1 beta Rabbit mAb
Product:IL1 beta Rabbit mAb
Journal:Biomed Res Int
Application:WB
IF:2.27
Species:Homo sapiens
PMID:32714980
Title:CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis

References for IL1 beta Rabbit mAb
Product:IL1 beta Rabbit mAb
Journal:Scand J Immunol
Application:WB
IF:2.71
Species:Mus musculus
PMID:33031600
Title:T cell immunoglobulin and mucin domain protein 3 inhibits glycolysis in RAW 264.7 macrophages through Hexokinase 2

References for IL1 beta Rabbit mAb
Product:IL1 beta Rabbit mAb
Journal:Inflammation
Application:IHC
IF:3.21
Species:Homo sapiens
PMID:33140204
Title: The Effect of Porphyromonas gingivalis Lipopolysaccharide on the Pyroptosis of Gingival Fibroblasts