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K-ras

Cat Number: MAB-94415
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human KRAS
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 -1:1000
Immunohistochemistry: 1:50 -1:200
Immunofluorescence: 1:50 -1:200
Immunoprecipitation: 1:50 -1:200

Molecular: 22kDa
Purification: Aff. Pur.
Synonyms: KRAS; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2; c-Ki-ras2; GTPase Kras
Background:

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles
MAB-94415-1.jpg

Western blot analysis of extracts of various cell lines, using KRAS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico.
Exposure time: 90s.

MAB-94415-2.jpg

Immunofluorescence analysis of C6 cells using KRAS antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

MAB-94415-3.jpg

Immunofluorescence analysis of NIH/3T3 cells using KRAS antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using KRAS antibody at dilution of 1:100. Blue: DAPI for nuclear staining