KU70 Monoclonal Antibody
Cat Number: | MAB-94696 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | EPR4027 |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Immunogen A synthetic peptide corresponding to a sequence within amino acids 500-609 of human Ku70. |
Reactivity: | Human, Mouse, Rat |
Applications: | Western Blot: 1:500 – 1:2000 |
Molecular Weight: | 70KDa |
Purification: | Aff. Pur. |
Synonyms: | CTC75; CTCBF; G22P1; KU70; ML8; TLAA |
Background: | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of |
Form: | Liquid |
Buffer: | PBS with 0.01% thiomersal,0.05% BSA,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Ku70 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.
Immunofluorescence analysis of NIH-3T3 cells using Ku70 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Ku70 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for
nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Ku70 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.