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KU70 Monoclonal Antibody

Cat Number: MAB-94696
Conjugate: Unconjugated
Size: 100 ug
Clone: EPR4027
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Immunogen A synthetic peptide corresponding to a sequence within amino acids 500-609 of human Ku70.
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: HC 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200 Immunocytochemistry CC 1:50 – 1:200
Molecular Weight: 70KDa
Purification: Aff. Pur.
Synonyms: CTC75; CTCBF; G22P1; KU70; ML8; TLAA
Background:

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of
nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
Form: Liquid
Buffer: PBS with 0.01% thiomersal,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
MAB-94696-1.jpg

Western blot analysis of extracts of various cell lines, using Ku70 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 1s.

MAB-94696-2.jpg

Immunofluorescence analysis of NIH-3T3 cells using Ku70 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

MAB-94696-3.jpg

Immunofluorescence analysis of NIH-3T3 cells using Ku70 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for
nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Ku70 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.