KU80
| Cat Number: | AB-11718 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of human KU86 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 -1:2000 |
| Molecular Weight: | 86kDa |
| Purification: | Purified |
| Synonyms: | KU86; KARP-1; KARP1; KU80; KUB2; Ku86; NFIV;X-ray repair cross complementing 5 |
| Background: | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein KU86. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA doublestrand break by non-homologous end joining and the completion of V(D)Jrecombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles |
Western blot analysis of extracts of various cell lines, using KU86 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human breast cancer using KU86 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KU86 Antibody at dilution of 1:100 (40x lens).