Top

Laminin Beta-2 Rabbit Polyclonal Antibody

Cat Number: AB-E97720
Conjugate: unconjated
Size: 100 ug
Clone: Polyclonal
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: The antiserum was produced against synthesized peptide derived from human LAMB2. AA range:61-110
Reactivity: Human, Mouse, Rat
Applications:

Western Blot: 1/500 – 1/2000.
Immunohistochemistry: 1/100 – 1/300.
Molecular Weight: 210kD
Purification: affinity purified
Synonyms: LAMB2; LAMS; Laminin subunit beta-2; Laminin B1s chain; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin sub
Background:

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth andmetastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure
consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1.
Form: Liquid
Buffer: PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
AB-E97720-1

Western blot analysis of lysates from RAW264.7 cells, using LAMB2 Antibody. The lane on the right is blocked
with the synthesized peptide.

AB-E97720-2

Western blot analysis of the lysates from HUVECcells using PMS2 antibody.

AB-E97720-3

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe