LL-37
| Cat Number: | AB-84264 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 31-170 of human CAMP |
| Reactivity: | Hu, Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 15kDa |
| Purification: | Aff. Pur. |
| Synonyms: | CAMP;CAP-18;CAP18;CRAMP;FALL-39;FALL39;HSD26;LL37 |
| Background: | This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using LL-37 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry of paraffin embedded rat bone marrow using
LL-37 antibody at dilution
of 1:200 (40x lens).
Immunohistochemistry of paraffin embedded human appendix using
LL-37 antibody at dilution of
1:200 (40x lens).
Immunofluorescence analysis of rat bone marrow using LL-37 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.