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LL-37

Cat Number: AB-84264
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 31-170 of human CAMP
Reactivity: Hu, Ms,Rt
Applications:

Western Blot: 1:500 – 1:2000
Immunohistochemistry: 1:50 – 1:200
Immunofluorescence: 1:50 – 1:200

Molecular: 15kDa
Purification: Aff. Pur.
Synonyms: CAMP;CAP-18;CAP18;CRAMP;FALL-39;FALL39;HSD26;LL37
Background:

This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84264-1.jpg

Western blot analysis of extracts of various cell lines, using LL-37 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

AB-84264-2.jpg

Immunohistochemistry of paraffin embedded rat bone marrow using
LL-37 antibody at dilution
of 1:200 (40x lens).

AB-84264-3.jpg

Immunohistochemistry of paraffin embedded human appendix using
LL-37 antibody at dilution of
1:200 (40x lens).

AB-84264-4.jpg

Immunofluorescence analysis of rat bone marrow using LL-37 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.