|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 31-170 of human CAMP|
Western Blot: 1:500 – 1:2000
This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation.
|Buffer:||PBS with 0.02% sodium azide,50% glycerol,pH7.3.|
|Storage:||Store at -20℃. Avoid freeze / thaw cycles.|
Western blot analysis of extracts of various cell lines, using LL-37 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemistry of paraffin embedded rat bone marrow using
LL-37 antibody at dilution
of 1:200 (40x lens).
Immunohistochemistry of paraffin embedded human appendix using
LL-37 antibody at dilution of
1:200 (40x lens).
Immunofluorescence analysis of rat bone marrow using LL-37 antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.