MAP1LC3b
| Cat Number: | AB-83629 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 |
| Molecular Weight: | 14kDa |
| Purification: | Purified |
| Synonyms: | ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a |
| Background: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol pH 7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 30s..
Western blot analysis of extracts of 293T cells, using LC3B antibody at 1:1000 ilution.293Tcells were treated by hloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution.Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit Exposure time: 180s.
Immunohistochemistry of paraffinembedded
rat brain using LC3B antibody
at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using LC3B Rabbit pAb at dilution of 1:100 (40x lens).293T cells were treated by Chloroquine (50 μM) at 37℃ for 20
hours.
Blue: DAPI for nuclear staining.