MAP1LC3b
| Cat Number: | AB-84132 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 16kDa |
| Purification: | Purified |
| Synonyms: | MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta |
| Background: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of NIH/3T3 cells,
using LC3B antibody at 1:1000 dilution. NIH/3T3
cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti- Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane
Blocking buffer: 3% nonfat dry milk inTBST
Detection: ECL West Pico Plus
Exposure time: 3min.
Immunofluorescence analysis of C6
cells using LC3B antibody at
dilution of 1:100. C6 cells were
treated by Chloroquine (50 μM) for 20
hours(left). Blue: DAPI for nuclear
staining.
Immunofluorescence analysis of
NIH/3T3 cells using LC3B antibody
at dilution of 1:100. NIH/3T3
cells were treated by Chloroquine (50
μM) for 20 hours(left). Blue: DAPI for
nuclear staining.
Immunofluorescence analysis of HeLa
cells using LC3B antibody at
dilution of 1:100. Hela cells were
treated by Chloroquine (50 μM) for 20
hours(left). Blue: DAPI for nuclear
staining.