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MAP1LC3b

Cat Number: AB-84132
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500 – 1:1000
Immunohistochemistry: 1:50 – 1:100
Immunofluorescence: 1:50 – 1:100

Molecular: 16kDa
Purification: Purified
Synonyms: MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta
Background:

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-84132-1.jpg

Western blot analysis of extracts of NIH/3T3 cells,
using LC3B antibody at 1:1000 dilution. NIH/3T3
cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti- Rabbit IgG
(H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane
Blocking buffer: 3% nonfat dry milk inTBST
Detection: ECL West Pico Plus
Exposure time: 3min.

AB-84132-2.jpg

Immunofluorescence analysis of C6
cells using LC3B antibody at
dilution of 1:100. C6 cells were
treated by Chloroquine (50 μM) for 20
hours(left). Blue: DAPI for nuclear
staining.

AB-84132-3.jpg

Immunofluorescence analysis of
NIH/3T3 cells using LC3B antibody
at dilution of 1:100. NIH/3T3
cells were treated by Chloroquine (50
μM) for 20 hours(left). Blue: DAPI for
nuclear staining.

AB-84132-4.jpg

Immunofluorescence analysis of HeLa
cells using LC3B antibody at
dilution of 1:100. Hela cells were
treated by Chloroquine (50 μM) for 20
hours(left). Blue: DAPI for nuclear
staining.