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MAP1LC3b

Cat Number: AB-83629
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Reactivity: Hu, Ms, Rt
Applications:

Western Blot: 1:500-1:2000
Immunohistochemistry (paraffin-embedded tissues): 1:50-1:200
Immunofluorescence: 1:20-1:200

Molecular: 14kDa
Purification: Purified
Synonyms: ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a
Background:

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Form: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol pH 7.3
Storage: Store at -20℃. Avoid freeze / thaw cycles.
AB-83629-1.jpg

Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 30s..

AB-83629-2.jpg

Western blot analysis of extracts of 293T cells, using LC3B antibody at 1:1000 ilution.293Tcells were treated by hloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution.Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit Exposure time: 180s.

AB-83629-3.jpg

Immunohistochemistry of paraffinembedded
rat brain using LC3B antibody
at dilution of 1:100 (40x lens).

AB-83629-4.jpg

Immunofluorescence analysis of 293T cells using LC3B Rabbit pAb at dilution of 1:100 (40x lens).293T cells were treated by Chloroquine (50 μM) at 37℃ for 20
hours.
Blue: DAPI for nuclear staining.