MRE11 Rabbit Polyclonal Antibody

Cat Number: AB-84200
Conjugate: Unconjugated
Size: 100ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-485 of human MRE11
Reactivity: Human, Rat

Western Blot: 1:500 – 1:1000
Immunoprecipitation: 1:500 – 1:1000

Molecular Weight: 81KDa
Purification: Affinity purification
Synonyms: MRE11;ATLD;HNGS1;MRE11A;MRE11B

This gene encodes a nuclear protein involved in homologous recombination, telomerelength maintenance, and DNA double-strand break repair. By itself, the protein has 3′ to 5′ exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3′ to 5′ exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Form: Liquid
Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell lines, using MRE11 antibody at 1:1000
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s.


Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MRE11 antibody.
Western blot was performed from the immunoprecipitate using MRE11 antibody at a
dilution of 1:1000.