MUCIN 5AC
| Cat Number: | AB-84263 |
|---|---|
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence at the N-terminus of human Mucin 5AC(552-567aa TMQLFMQLAPKLRGQT). |
| Reactivity: | Hu |
| Applications: | Western blot: 0.1-0.5μg/ml |
| Molecular Weight: | 585570 MW |
| Purification: | Aff. Pur. |
| Background: | Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease(COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a direct and critical mediator of resistance during intestinal nematode infection. |
| Form: | Liquid |
| Buffer: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage: | At -20°C for one year. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
Anti-Mucin 5AC antibody, All Western blotting
All lanes: Anti-MUC5AC at0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 527KD
Observed bind size: 300KD
Figure 2. IHC analysis of Mucin 5AC using anti-Mucin 5ACantibody.
Mucin 5AC was detected in paraffin-embedded section o fhuman gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitoperetrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Mucin 5AC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG
was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.