N-MYC
| Cat Number: | AB-84269 |
|---|---|
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 49kDa |
| Purification: | Aff. Pur. |
| Synonyms: | MODED;N-myc;NMYC;ODED;bHLHe37;MYCN |
| Background: | This gene is a member of the MYC family and encodes a protein with a basic helixloop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using MYCN
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 90s.
Immunohistochemistry of paraffin embedded mouse brain using MYCN
antibody (40x lens).
Immunohistochemistry of paraffin
embedded rat brain using MYCN
antibody (40x lens).
Formalin-fixed and paraffin embedded human colon carcinoma labeled with
Anti-n-Myc Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.