NANOG
| Cat Number: | AB-84338 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Nanoghomeobox |
| Reactivity: | Hu,Ms |
| Applications: | Western Blot: 1:500-1:2000 |
| Molecular Weight: | 43-48 kDa |
| Purification: | Aff. Pur. ≥95% as determined by SDS-PAGE |
| Background: | Transcription regulator involved in inner cell mass and embryonic stem(ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5′-TAAT[GT][GT]-3′ or 5′-[CG][GA][CG]C[GC]ATTAN[GC]-3′. Able to autorepress its expression in differentiating(ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 |
| Storage: | T -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
rat brain tissue were subjected to SDS PAGE followed by western blot with NANOG Antibody at dilution of 1:2000
Immunofluorescent analysis of NCCIT cells, using Nanog
Antibody.