NEFL Mouse Monoclonal Antibody
| Cat Number: | MABN82586 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ul |
| Concentration: | 1mg/ml |
| Host: | Mouse |
| Isotype: | Mouse IgG1 |
| Immunogen: | Purified recombinant fragment of human NEFL (AA: 1-200) expressed in E. Coli. |
| Reactivity: | Human,Mouse,Rat,Rabbit |
| Applications: | WB 1:500-1:2000,IHC 1:100-1:500,ELISA 1:5000-1:20000,FC 1:200-1:400 |
| Molecular: | 5kDa |
| Purification: | Affinity purification |
| Synonyms: | NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110 |
| Background: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| Form: | liquid |
| Buffer: | Purified antibody in PBS with 0.05% sodium azide |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using NEFL mouse mAb against HEK293 (1) cell lysate.
Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).