Nestin Rabbit Polyclonal Antibody
| Cat Number: | AB-84781 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | nestin |
| Applications: | Western Blot: 1:500-1:2000 |
| Molecular Weight: | 170 kDa |
| Purification: | Immunogen affinity purified |
| Synonyms: | NES, Nestin |
| Background: | Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 |
| Storage: | Store at -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
MCF7 cells were subjected to SDS PAGE followed by western blot with NES antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse brain using NES antibody at dilution of 1:50
Immunofluorescent analysis of SH-SY5Y cells using NES antibody at dilution of 1:50.