Nestin Rabbit Polyclonal Antibody

Cat Number: AB-84781
Conjugate: Unconjugated
Size: 100 ug
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: nestin

Western Blot: 1:500-1:2000
Immunohistochemistry: 1:500-1:200
Immunofluorescence: 1:20-1:200

Molecular Weight: 170 kDa
Purification: Immunogen affinity purified
Synonyms: NES, Nestin

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Form: Liquid
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Storage: Store at -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

MCF7 cells were subjected to SDS PAGE followed by western blot with NES antibody at dilution of 1:1000


Immunohistochemistry of paraffin-embedded mouse brain using NES antibody at dilution of 1:50


Immunofluorescent analysis of SH-SY5Y cells using NES antibody at dilution of 1:50.