NME
| Cat Number: | AB-81311 |
|---|---|
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1 |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 21kDa |
| Purification: | Aff. Pur. |
| Synonyms: | NME1; AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; nucleoside diphosphate kinase A |
| Background: | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ‘A’ (encoded by this gene) and ‘B’ (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Cotranscription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of
various cell lines, using NME1
antibody.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) ) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Immunohistochemistry of paraffinembedded
mouse brain using NME1
Antibody at dilution of 1:200
(40x lens).
Immunohistochemistry of paraffinembedded
rat heart using NME1
Antibody at dilution of 1:200
(40x lens).
Immunohistochemistry of paraffinembedded
rat heart using NME1
Antibody at dilution of 1:200
(40x lens).
Immunofluorescence analysis of C6
cells using NME1 antibody at
dilution of 1:100. Blue: DAPI for
nuclear staining.
Immunofluorescence analysis of HeLa
cells using NME1 antibody at
dilution of 1:100. Blue: DAPI for nuclear
staining.