NOTCH 1 Rabbit Polyclonal Antibody

Cat Number: AB-90186
Conjugate: Unconjugated
Size: 100μg
Clone: POLY
Concentration: 1mg/ml
Host: Rabbit
Isotype: IgG
Immunogen: Immunogen A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human Notch1.
Reactivity: Human, Mouse

Western Blot: 1:500 – 1:2000
Immunoprecipitation: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Molecular Weight: 125kDa
Purification: Affinity purification
Synonyms: hN1; AOS5; TAN1; AOVD1; Notch1

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types.
Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, Tcell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.

Form: Liquid
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of various lysates using Notch1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.


Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg Notch1 antibody. Western blot was performed from the immunoprecipitate using Notch1 antibody at a dilution of 1:1000.