NOX1
| Cat Number: | AB-84209 |
|---|---|
| Size: | 100μg |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide from human Nox1 protein. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-2000 |
| Molecular Weight: | 65kDa |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity chromatography using specific immunogen. |
| Synonyms: | NADPH oxidase 1 MOX-1 NADH/NADPH mitogenic oxidase subunit P65-MOX NOH-1. |
| Background: | Specificity: The antibody detects endogenous Nox1. Tissue Specificity: NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. |
| Form: | Liquid |
| Buffer: | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage: | Store at -20°C, and avoid repeat freeze-thaw cycles. |
Western blot analysis of 293T Hela VEC KB mouse-kidney 3T3 lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000.
Western blot analysis of extracts of
various cell lines, using NOX1
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.
Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.