NRF2 Rabbit Monoclonal Antibody
Cat Number: | MAB-94130 |
---|---|
Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | SP611 |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Recombinant protein of human NRF2. |
Reactivity: | Hu, Ms,Rt |
Applications: | Western Blot: 1:1000 |
Molecular Weight: | 97-100kDa |
Purification: | Aff. Pur. |
Background: | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals . Multiple transcript variants encoding different isoforms have been characterized for this gene. |
Form: | Liquid |
Buffer: | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
![MAB-94130-1.jpg](https://immunologicalsciences.com/wp-content/uploads/2022/01/MAB-94130-1.jpg)
Western Blot – NFE2L2 antibody
of various cell lines, using NRF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer:3% nonfat dry milk in TBST
Detection: ECL West Pico Plus Kit
Exposure time: 60s.
![MAB-94130-2.jpg](https://immunologicalsciences.com/wp-content/uploads/2022/01/MAB-94130-2.jpg)
Immunohistochemistry –
NFE2L2 antibody of paraffin-embedded mouse leydig cells – dilution 1:100
![MAB-94130-3.jpg](https://immunologicalsciences.com/wp-content/uploads/2022/01/MAB-94130-3.jpg)
Immunohistochemistry – NFE2L2 antibody of paraffin-embedded mouse kidney – dilution 1:100
![MAB-94130-4.jpg](https://immunologicalsciences.com/wp-content/uploads/2022/01/MAB-94130-4.jpg)
Immunofluorescence analysis of HeLa cells using NFE2L2 antibody using at dilution of 1:100. Blue: DAPI for nuclear staining.