NRF2 Rabbit Monoclonal Antibody
| Cat Number: | MAB-94130 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | SP611 |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605 |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:1000 |
| Molecular Weight: | 75-100kDa |
| Purification: | Aff. Pur. |
| Background: | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals . Multiple transcript variants encoding different isoforms have been characterized for this gene. |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western Blot – NFE2L2 antibody
of various cell lines, using NRF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer:3% nonfat dry milk in TBST
Detection: ECL West Pico Plus Kit
Exposure time: 60s.
Immunohistochemistry –
NFE2L2 antibody of paraffin-embedded mouse leydig cells – dilution 1:100
Immunohistochemistry – NFE2L2 antibody of paraffin-embedded mouse kidney – dilution 1:100
Immunofluorescence analysis of HeLa cells using NFE2L2 antibody using at dilution of 1:100. Blue: DAPI for nuclear staining.