NRF2 Rabbit Monoclonal Antibody

Cat Number: MAB-94130
Conjugate: Unconjugated
Size: 100 ug
Clone: SP611
Concentration: 1mg/ml
Host: Rb
Isotype: IgG
Immunogen: Recombinant protein of human NRF2.
Reactivity: Hu, Ms,Rt

Western Blot: 1:1000
Immunohistochemistry (paraffin tissues): 1:50 – 1:200
Immunocytochemistry: 1:50 – 1:200
Immunofluoresence: 1:50 – 1:200

Molecular Weight: 97-100kDa
Purification: Aff. Pur.

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals . Multiple transcript variants encoding different isoforms have been characterized for this gene.

Form: Liquid
Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage: Store at -20℃. Avoid freeze / thaw cycles.

Western Blot – NFE2L2 antibody
of various cell lines, using NRF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer:3% nonfat dry milk in TBST
Detection: ECL West Pico Plus Kit
Exposure time: 60s.


Immunohistochemistry –
NFE2L2 antibody of paraffin-embedded mouse leydig cells – dilution 1:100


Immunohistochemistry – NFE2L2 antibody of paraffin-embedded mouse kidney – dilution 1:100


Immunofluorescence analysis of HeLa cells using NFE2L2 antibody using at dilution of 1:100. Blue: DAPI for nuclear staining.