p53 Rabbit Polyclonal Antibody
| Cat Number: | AB-J11971 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p53. AA range:10-59 |
| Reactivity: | Human;Mouse;Rat;Monkey |
| Applications: | Western Blot: 1/500 – 1/2000. |
| Molecular Weight: | 53kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Synonyms: | TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53 |
| Background: | tumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded proteinresponds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni |
| Form: | Liquid |
| Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Western blot analysis of lysates from 1)p53 knockout A431 cell , 2)A431 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Anti
Immunofluorescence analysis of human-liver tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibodywas diluted at 1:300(room temperature, 50min).3, PictureB: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-liver tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture
Immunofluorescence analysis of human-lung tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody
was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B