P70-S6K (RPS6KB1)
| Cat Number: | AB-83588 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human P70 S6K. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 70kDa |
| Purification: | Aff. Pur. |
| Synonyms: | PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha |
| Background: | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using p70 S6 Kinase 1 1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 5s
Immunohistochemistry of paraffin embedded
human breast cancer using p70 S6 Kinase 1 1 Rabbit pAb at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using p70 S6 Kinase 1 1 Rabbit pAb at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.