PARK2
| Cat Number: | AB-84037 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human Parkin |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot 1:100 – 1:500 |
| Molecular Weight: | 52 kDa |
| Purification: | Affinity Purified |
| Synonyms: | PDJ; AR-JP; LPRS2; PARKIN |
| Background: | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. |
| Form: | Liquid |
| Buffer: | PBS with 0.05% proclin300,50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using Parkin antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s
Western blot analysis of extracts of 293T cells, using Parkin antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse colon using Parkin Rabbit pAb at dilution of 1:100 (40x lens).
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol
Immunohistochemistry analysis of paraffin-embedded rat testis using Parkin Rabbit pAb at dilution of 1:100
(40x lens).
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.