|Immunogen:||Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Parkin.|
Western Blot: 1:500 – 1:2000
Background: The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.
|Buffer:||Liquid in PBS containing 0.02% sodium azide, 50% glycerol, Ph7.3.|
|Storage:||At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles.|
Western blot on Mouse cells
Western blot analysis of extracts of various cell lines, using Parkin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST
Immunofluorescence analysis of mouse cells using Parkin antibody