PCNA
| Cat Number: | AB-81598 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA. |
| Reactivity: | HU, Ms, Rt |
| Applications: | Western Blot: 1:500 – 1:2000 |
| Molecular Weight: | 36kDa |
| Purification: | Purified |
| Synonyms: | PCNA;ATLD2 |
| Background: | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Immunohistochemistry of paraffinembedded
rat liver using PCNA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffinembedded
human breast cancer using
PCNA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffinembedded
mouse testis using PCNA
antibody at dilution of 1:100 (40xlens).
Immunofluorescence analysis of HeLa cells
using PCNA antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.