PCNA
Cat Number: | AB-81598 |
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Conjugate: | Unconjugated |
Size: | 100 ug |
Clone: | POLY |
Concentration: | 1mg/ml |
Host: | Rb |
Isotype: | IgG |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA. |
Reactivity: | HU, Ms, Rt |
Applications: | Western Blot: 1:500 – 1:2000 |
Molecular: | 36kDa |
Purification: | Purified |
Synonyms: | PCNA;ATLD2 |
Background: | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
Form: | Liquid |
Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.
Immunohistochemistry of paraffinembedded
rat liver using PCNA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffinembedded
human breast cancer using
PCNA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffinembedded
mouse testis using PCNA
antibody at dilution of 1:100 (40xlens).
Immunofluorescence analysis of HeLa cells
using PCNA antibody at dilution of 1:100.
Blue: DAPI for nuclear staining.