PD-1 Rabbit Polyclonal Antibody
| Cat Number: | AB-84286 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-259 of human PD-1/CD279. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | Western Blot: 1:500 – 1:1000 Immunocytochemistry: 1:50 – 1:200 |
| Molecular Weight: | 50kDa/35kDa |
| Purification: | Affinity purification |
| Synonyms: | PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD-1/CD279 |
| Background: | Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of various lysates using PD-1/CD279 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 1s.
Western blot analysis of various lysates using PD-1/CD279 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded human tonsil using PD-1/CD279 Rabbit pAb at dilution of 1:100 (40xlens). Secondary antibody: Cy3-
conjugated Goat anti-Rabbit IgG(H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.