PDE4D
| Cat Number: | AB-83940 |
|---|---|
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 249-518 of human PDE4D |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 -1:1000 |
| Molecular Weight: | 91kDa, 105kDa |
| Purification: | Aff. Pur. |
| Synonyms: | PDE4D; ACRDYS2; DPDE3; HSPDE4D; PDE43; PDE4DN2 ; STRK1; phosphodiesterase 4D |
| Background: | This gene encodes one of four mammalian counterparts to the fruit fly ‘dunce’ gene. The encoded protein has 3′,5′-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of rat brain, using PDE4D antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using PDE4D antibody at dilution of 1:100 (40x lens).
WB strong expose, strong ECL
WB weak expose