PDGFR Alpha Rabbit Polyclonal Antibody
| Cat Number: | AB-84772 |
|---|---|
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Clone: | POLY |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 896-1059 of human PDGFR alpha |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 – 1:1000 |
| Molecular Weight: | 150-190kDa |
| Purification: | Affinity purification |
| Synonyms: | CD140A; PDGFR2; PDGFR-2 |
| Background: | This gene encodes a cell surface tyrosine kinase receptor for members of the plateletderived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. |
| Form: | Liquid |
| Buffer: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20℃. Avoid freeze / thaw cycles. |
Western blot analysis of extracts of various cell lines, using PDGFR alpha antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.
Immunofluorescence analysis of U2OS using PDGFR antibody at dilution of 1:100 (40x lens).
Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse heart using PDGFR alpha Rabbit pAb at
dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing
with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat spleen using PDGFR alpha Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.